Composite

Part:BBa_K2073011

Designed by: WEI-CHIA HSU   Group: iGEM16_NTHU_Taiwan   (2016-10-14)


CRCB(bpe) with IPTG promoter

Excess fluoride is toxic for bacteria. After the degradation of PFOA, fluoride would be released simultaneously. To prevent the E. coli killed by excess fluoride, we transfected Crcb(bpe) gene into E. Coli.
Crcb(bpe) is one of fluoride transporters - integration with the plasma membrane - able to expulse fluoride from E. Coli.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 423
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 415
    Illegal AgeI site found at 443
    Illegal AgeI site found at 572
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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