Composite

Part:BBa_K2073007

Designed by: YU-CHUN LIN   Group: iGEM16_NTHU_Taiwan   (2016-10-14)


kit-3 facdex3+GFP

this part contain BBa_J04500(IPTG promoter+RBS)+BBa_K2073001(fac-dex3)+BBa_E0040(GFP)+BBa_B0012(ter) We transform this biobrick into E.coli strain BL21, and use IPTG promoter to control the fac-dex1 protein express. When this engineered bacteria be induced by IPTG overnight (at least 12 hours), it would show GFP fluorescence. Then we know the fac-dex1 is expressed by bacteria.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 829
    Illegal NgoMIV site found at 1162
    Illegal AgeI site found at 1190
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1947


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