Coding

Part:BBa_K2071954

Designed by: Pavle Jeremic   Group: iGEM16_UCSC   (2016-10-14)


A non-specific phosphotransferase, codon optimized for B.subtilis.

This part codes for a phosphotransferase that is able to de-phosphorylate Erythrose-4-Phosphate. It is not a highly specific phosphotransferase and while it operates on both Erythrose-4-Phosphate, its original designation as a beta-phosphoglucomutase indicates it is relatively non-specific. This may pose an issue for cellular growth. It is recommended to grow the bacteria at a lower temperature to determine if non-specific de-phosphorylation is an issue.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None