Generator

Part:BBa_K2070027

Designed by: iGEM UT-Tokyo 2016   Group: iGEM16_UT-Tokyo   (2016-10-14)


Cell cycle dependent GFPlva reporter

This part contains a GFPlva coding region ligated downstream of promoter Pnrd (BBa_K847211). During cell division, the promoter is activated, generating GFPlva.

Reference

Sun, L., & Fuchs, J. A. (1992, 10). Escherichia coli ribonucleotide reductase expression is cell cycle regulated. Molecular Biology of the Cell, 3(10), 1095-1105. doi:10.1091/mbc.3.10.1095 Sun, L., Jacobson, B. A., Dien, B. S., Srienc, F., & Fuchs, J. A. (1994). Cell cycle regulation of the Escherichia coli nrd operon: requirement for a cis-acting upstream AT-rich sequence. Journal of Bacteriology, 176(8), 2415–2426.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 706


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Categories
Parameters
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