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Part:BBa_K2069004

Designed by: Mara Valverde Rascón   Group: iGEM16_Tec-Chihuahua   (2016-10-12)


pT7 + RBS + Reflectin (+LipA tag) + RBS + Cpn10 + RBS + Cpn60 + RBS + CspC + RBS + Antifungal GAFP-1

This is team Tec-Chihuahua gene expression generator. It expresses the chaperonins of the Cpn10/Cpn60 system for expression of proteins in atypical temperatures as well as the CspC protein, all with a strong RBS and pT7 promoter for high expression. It also expresses the GAFP-1 from Gastrodia and contains a t1 terminator as well as the early T7 phage terminator.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2112
    Illegal BamHI site found at 2178
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 3180
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 971


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