Generator

Part:BBa_K2068005

Designed by: Nithin Dharmaraj   Group: iGEM16_UCLA   (2016-09-13)


O3-33 Wild Type

The O3-33 gene codes for the production of one subunit of a protein cage. Each subunit consists of a trimeric protein that is designed to have low-energy protein-protein interfaces. Expression of this subunit will lead to the self assembly of a octahedral structured protein cage.Protein cages, are self assembling supramolecular protein structures where multiple protein subunits interact non covalently to form a higher order structure. This protein cage, O3-33, is a 24 subunit, 13nm diameter cage.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 591
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 284
    Illegal AgeI site found at 213
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//awards/part_collection/2016
Parameters
None