Part:BBa_K2068000
PCQuad 3.0 Wildtype
The PCQuad gene codes for the production of one subunit of a protein cage. Each subunit is made up of a dimer protein(M1 Matrix) and a trimer protein(Bromoperoxidase) fused by an alpha helical linker. Expression of this subunit will lead to the self assembly of a tetrahedral structured protein cage. Protein cages, are self assembling supramolecular protein structures where multiple protein subunits interact non covalently to form a higher order structure. Our protein cage, PCquad, is a 12 subunit cage where each subunit is a fusion of two naturally oligomerizing proteins -- a trimer(Bromoperoxidase) and a dimer(M1 Matrix). The alpha helical fusion protein that links the trimer and dimer fixes them in a very specific orientation allowing for self assembly once subunits interact with each other.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 408
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 105
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 768
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