Coding

Part:BBa_K2066546

Designed by: Kalen Clifton, Christine Gao, Andrew Halleran, Ethan Jones, Likhitha Kolla, Joseph Maniaci, John Marken, John Mitchell, Callan Monette, Adam Reiss   Group: iGEM16_William_and_Mary   (2016-10-14)


Synthetic Enhancer Project: glnG (NRI)

Sequence from Amit, R., Garcia, H. G., Phillips, R. & Fraser, S. E. Building enhancers from the ground up: a synthetic biology approach. Cell146, 105–118 (2011).

NRI coding region. NRI will get phosophorylated by NRII2302 kinase and this activated version can bind to the enhancer and when the DNA forms a loop, it can bind to the promoter poised with a sigma 54 RNA poly and initiate transcription.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 519
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None