Measurement

Part:BBa_K2066536

Designed by: John Marken   Group: iGEM16_William_and_Mary   (2016-10-14)


Spinach Aptamer w/ tRNA Scaffold

Spinach aptamer sequence is taken from Pothoulakis et al. 2013 (“The Spinach RNA Aptamer as a Characterization Tool for Synthetic Biology”). The mCherry sequence is taken from WM16_029 with the start codon and extra stop codon added. Spinach aptamer can be used for relative fluorescent abundance measurement at the RNA level.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 120
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 120
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 120
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 120
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None