Generator

Part:BBa_K2066533

Designed by: John Marken   Group: iGEM16_William_and_Mary   (2016-10-14)


tetO Synthetic Enhancer Helper Cassette

This is the pACT-Tet sequence from Amit et al. 2011 “Building Synthetic Enhancers from the Ground Up” paper. This is meant for use in Tet based synthetic enhancer systems.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 929
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 2303
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None