DNA

Part:BBa_K2066531

Designed by: Kalen Clifton, Christine Gao, Andrew Halleran, Ethan Jones, Likhitha Kolla, Joseph Maniaci, John Marken, John Mitchell, Callan Monette, Adam Reiss   Group: iGEM16_William_and_Mary   (2016-10-14)


Synthetic Enhancer Project: 3X TetO Binding Cassette(52S)

52S binding cassette has three TetO binding sites. Based on the amount of aTc in the system, which binds and inactivates TetR repressor, the amount of Tetr bound to these two TetO varies and thus flexibility of the DNA is also changing and ultimately the looping between the enhancer and promoter and output levels. These three TetO sites on 52S allows for four discrete states: unrepressed, two intermediate steps, completely repressed. This part is from a plasmid gifted by Amit et. al 2011.

Source: Amit, R., Garcia, H. G., Phillips, R. & Fraser, S. E. Building enhancers from the ground up: a synthetic biology approach. Cell146, 105–118 (2011).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 24
    Illegal NheI site found at 119
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 84
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None