Coding

Part:BBa_K2066109

Designed by: Kalen Clifton, Christine Gao, Andrew Halleran, Ethan Jones, Likhitha Kolla, Joseph Maniaci, John Marken, John Mitchell, Callan Monette, Adam Reiss   Group: iGEM16_William_and_Mary   (2016-10-08)


pTet GFP + TetR (weakened)

This part combines a pTet GFP reporter (BBa_K2066023) and a constitutive tetR expression construct (BBa_K2066022) onto the same plasmid, with a modified RBS on the tetR expression construct in order to replicate the effect of having the tetR construct be on a lower-copy plasmid.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1004
    Illegal NheI site found at 1027
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 764


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Categories
Parameters
None