Composite

Part:BBa_K2066033

Designed by: Kalen Clifton, Christine Gao, Andrew Halleran, Ethan Jones, Likhitha Kolla, Joseph Maniaci, John Marken, John Mitchell, Callan Monette, Adam Reiss   Group: iGEM16_William_and_Mary   (2016-10-07)


pBad - RFP on UNS

This part is used to test the induction of the pBad promoter. Its insert is the I0050 functional RFP cassette from the 2016 Distribution Kit, BBa_K577882, but flanked by UNS2 (BBa_K2066018) and UNS3 (BBa_K2066019) to put the insert onto the UNS backbone standard developed by William and Mary iGEM 2016.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1287
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1227
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1019
    Illegal AgeI site found at 1873
    Illegal AgeI site found at 1985
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1001


[edit]
Categories
Parameters
None