Composite
Part:BBa_K2064001
Designed by: Dragos S. Chiriac Group: iGEM16_Queens_Canada (2016-10-21)
TyrA Donor COM
We developed a BioBrick that is used to link the inserted protein with a protein containing a Tyrocidine B acceptor domain. This allows the two proteins to come together in physical space through protein-protein interactions and increase the efficiency of enzymatic gene clusters. It is under the control of an inducible T7 promoter and terminator pair from a pET-16b vector allowing expression of protein. It contains a golden gate cloning site for the scarless insertion of NRPS modules or other proteins.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 64
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 32
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 195
Illegal BsaI.rc site found at 174
[edit]
Categories
Parameters
None |