Composite

Part:BBa_K2064001

Designed by: Dragos S. Chiriac   Group: iGEM16_Queens_Canada   (2016-10-21)


TyrA Donor COM

We developed a BioBrick that is used to link the inserted protein with a protein containing a Tyrocidine B acceptor domain. This allows the two proteins to come together in physical space through protein-protein interactions and increase the efficiency of enzymatic gene clusters. It is under the control of an inducible T7 promoter and terminator pair from a pET-16b vector allowing expression of protein. It contains a golden gate cloning site for the scarless insertion of NRPS modules or other proteins.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 64
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 32
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 195
    Illegal BsaI.rc site found at 174


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