Plasmid

Part:BBa_K2061002

Designed by: avi matityahu   Group: iGEM16_Tel-Hai   (2016-08-23)


gRNA1 for editing F508 deletion

This plasmid contains the sequence coding for the gRNA for editing the CFTR F508 deletion under U6 promoter. The part was cloned into the pSB1C3 plasmid with chloramphenicol rresistance.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1
    Illegal XbaI site found at 431
    Illegal PstI site found at 466
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal PstI site found at 466
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
    Illegal BamHI site found at 49
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1
    Illegal XbaI site found at 431
    Illegal PstI site found at 466
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1
    Illegal XbaI site found at 431
    Illegal PstI site found at 466
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None