Plasmid

Part:BBa_K2061000

Designed by: avi matityahu   Group: iGEM16_Tel-Hai   (2016-08-23)


tGFP ORF under EF1a promoter

This plasmid contains the tGFP open reading frame under the control of human EF1a promoter. The part was cloned into the pSB1C3 plasmid with chloramphenicol rresistance.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 287
    Illegal PstI site found at 791
    Illegal PstI site found at 1223
    Illegal PstI site found at 1804
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 287
    Illegal PstI site found at 791
    Illegal PstI site found at 1223
    Illegal PstI site found at 1804
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 541
    Illegal XhoI site found at 939
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 287
    Illegal PstI site found at 791
    Illegal PstI site found at 1223
    Illegal PstI site found at 1804
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 287
    Illegal PstI site found at 791
    Illegal PstI site found at 1223
    Illegal PstI site found at 1804
    Illegal AgeI site found at 53
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1365


[edit]
Categories
//chassis/eukaryote/human
Parameters
color