Composite

Part:BBa_K2057004

Designed by: José Eleazar Barboza Corona   Group: iGEM16_Guanajuato_Mx   (2016-10-17)


LasR under the regulation of the promoter BtI-BtII

The promoter BtI-BtII from Bacillus thuringiensis regulate the expression of the cry genes during sporulation, but it is functional in Escherichia coli in a constitutive form. Promoter BtI-BtII also includes the ribosome binding site.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 715
    Illegal AgeI site found at 912
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//chassis/prokaryote
Parameters
biologyBacillus thuringiensis, Pseudomona aeruginosa, Escherichia coli