Composite

Part:BBa_K2057003

Designed by: José Eleazar Barboza Corona   Group: iGEM16_Guanajuato_Mx   (2016-10-17)


LasR under the regulation of the promoter chiA74

The promoter chiA74 will regulate the expression of the LasR in a constitutive form. The promoter chiA74 from Bacillus thuringiensis is functional in Escherichia coli, and regulate the expression of a gene in a constitutive form.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 489
    Illegal XbaI site found at 506
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 97
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 489
    Illegal XbaI site found at 506
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 489
    Illegal XbaI site found at 506
    Illegal NgoMIV site found at 896
    Illegal AgeI site found at 1093
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//chassis/prokaryote/ecoli
Parameters
biologyBacillus thuringiensis, Pseudomona aeruginosa, Escherichia coli