Part:BBa_K2057001

Promoter BtI-BtII

The BtI-BtII promoter (BtI-BtIIp) is a dual strong promoter that controls the expression of Cry1Ac proteins in Bacillus thuringiensis, but it allows the expression of a gene constitutively in Escherichia coli. In this sense, BtI-BtIIp can be used to allow the synthesis of a gene in E. coli that want to be expressed constitutively in this bacterium. This is one of the promoter that our team will use to express constitutively the lasR gene in E. coli. The biobrick also include a ribosome-binding site to to start the synthesis of a proteins. This promoter can can be used not only in E. coli but also in B. subtilis and B. thuringiensis. Promoter BtI-BtII has the consensus sequences in 285-291 bp (BtI, -35 region), 294-302 (Bt II, -10) and 306-313 (BtI, -10). Sequence also includes the ribosome binding site that is located on 377-381 bp. The whole sequence of the cry1Ac gene including its regulatory elements (promoter, RBS, transcriptional terminator) is found under accession number M35524 (GenBank nucleotide sequence database).

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]