Regulatory

Part:BBa_K2057000

Designed by: Uriel Eleazar Barboza Pérez   Group: iGEM16_Guanajuato_Mx   (2016-10-14)


Promoter chiA74 from Bacillus thuringiensis

The chiA74 (chiA74p) controls the expression of the endochitinase chiA74 from a strain of Bacillus thuringiensis. This regulatory element is useful to drive expression of a gene in Escherichia coli (Barboza-Corona et al. 2003, 2014). We have shown that chiA74p drive the expression of the green fluorescent (gfp) in E. coli. This is one of the promoter that our team will use to express the LasR in a constitutive form in E. coli . chiA74p has the promoter conserved sequence in 374-384 bp (-35 region) and 399-404 (-10). The whole sequence of the endochitinase chiA74, including its regulatory elements, is found in a GenBank data under the accession number AF424979.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 97
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//chassis/prokaryote/ecoli
Parameters
biologyBacillus thuringiensis