Part:BBa_K2056001

NsrR - NO(x) sensitive pYear, pNirk repressor protein

This part is the improved version of the part K248001. It contains the NsrR (NO(x) sensitive pYear, pNirk repressor protein) encoding region. Escherichia coli (E. coli) detects environmental nitrate by the yeaR-yoaG operon. PyeaR is regulated by the NsrR repressor protein. Nitric oxide binds to the NsrR protein and relieves the repression on PyeaR. As a result, any genes that are downstream of PyeaR are expressed. This part is also related with BBa_K2056002, the promoter region of the NirK protein, because NsrR activates the transcription of NirK under high nitrite conditions. Due to these properties, we can use this part as a sensor for oxides of nitrogen.

K248001 contained illegal sites that made it incompatible with all assembly standards (RFC 10,RFC 12,RFC 21, RFC 23,RFC 25,RFC 1000) and had not been submitted to the Registry. The 2016 Peshawar team removed these illegal sites, codon optimized this sequence for expression in E.coli and for synthesis, synthesized and submitted this new improved part K2056001 to the Registry.

The alignment file can be found here: File:BBa K248001, K2056001-alignment.pdf

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 154
  
• 1000
  COMPATIBLE WITH RFC[1000]