Composite

Part:BBa_K2055020

Designed by: Raul Garza & Carlos Vasquez   Group: iGEM16_Tec-Monterrey   (2016-09-28)


Overexpression of TetH with heat shock promoter and Inverter.

If the characterization of HS promoter + Inverter + RFP works, we will use this construct for our project, to recover Copper, Nickel and Zinc with Acidithiobacillus Ferrooxidans.

Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 237
    Illegal XhoI site found at 422
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2592
    Illegal AgeI site found at 377
    Illegal AgeI site found at 2956
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 158
    Illegal BsaI.rc site found at 374
    Illegal BsaI.rc site found at 2578


[edit]
Categories
Parameters
None