Composite

Part:BBa_K2055019

Designed by: Raul Garza & Carlos Vasquez   Group: iGEM16_Tec-Monterrey   (2016-09-28)


Part designed for verification and characterization of the heat the shock promoter and Inverter.

We used an existing RBS + RFP from iGEM parts. LacI and FUR proteins are optimized for Acidithiobacillus ferrooxidans. It has two RBS after the constitutive promoter and before the FUR CDS.

Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 237
    Illegal XhoI site found at 422
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 377
    Illegal AgeI site found at 2427
    Illegal AgeI site found at 2539
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 158
    Illegal BsaI.rc site found at 374


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Parameters
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