Part designed for verification and characterization of the heat the shock promoter and Inverter.
We used an existing RBS + RFP from iGEM parts. LacI and FUR proteins are optimized for Acidithiobacillus ferrooxidans. It has two RBS after the constitutive promoter and before the FUR CDS.
Sequence and Features
- 10COMPATIBLE WITH RFC
- 12COMPATIBLE WITH RFC
- 21Illegal BglII site found at 237
Illegal XhoI site found at 422
- 23COMPATIBLE WITH RFC
- 25Illegal AgeI site found at 377
Illegal AgeI site found at 2427
Illegal AgeI site found at 2539
- 1000Illegal BsaI site found at 158
Illegal BsaI.rc site found at 374