Coding

Part:BBa_K2055017

Designed by: Raul Garza & Carlos Vasquez   Group: iGEM16_Tec-Monterrey   (2016-09-28)


Tetrathionate Hydrolase (TetH) CDS with a RBS for overexpression in Acidithiobacillus ferrooxidans.

Acidithiobacillus ferrooxidans is a major participant in consortia of microorganisms used for bioleaching. It can obtain energy from the oxidation of Fe2+, H2, S0, and various reduced inorganic sulfur compounds (RISCs). Tetrathionate is a key intermediate during RISC oxidation, hydrolyzed by tetrathionate hydrolase (TetH), and used as sole energy source.

Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 738
    Illegal AgeI site found at 1102
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 724


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Categories
Parameters
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