Composite

Part:BBa_K2055016

Designed by: Raul Garza & Carlos Vasquez   Group: iGEM16_Tec-Monterrey   (2016-09-28)


Part designed for verification and characterization of the FUR/LacI inverter

E. coli constitutive promoter with Ferric Uptake Regulator & LacI Inverter. Activates RFP expression in presence of iron

Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 204
    Illegal XhoI site found at 389
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 344
    Illegal AgeI site found at 2394
    Illegal AgeI site found at 2506
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 125
    Illegal BsaI.rc site found at 341


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Categories
Parameters
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