Inverter

Part:BBa_K2055014

Designed by: Raul Garza & Carlos Vasquez   Group: iGEM16_Tec-Monterrey   (2016-09-28)


Inverter with Ferric Uptake Regulator & LacI. Activates gene expression in presence of iron.

Inverter with Ferric Uptake Regulator & LacI. Activates gene expression in presence of iron. LacI and FUR proteins are optimized for Acidithiobacillus ferrooxidans.

Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 140
    Illegal XhoI site found at 325
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 280
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 61
    Illegal BsaI.rc site found at 277


[edit]
Categories
Parameters
None