DNA
Active Com

Part:BBa_K2054006

Designed by: LAI HEI WAI   Group: iGEM16_Hong_Kong_HKU   (2016-10-14)


All Oligos for simplified Tetrahedral Nanostructure

This will be used to build and express our desired functional nanostructure in E. coli that target micro RNA biomarkers, but a simplified 2-strand nanostructure. This plasmid encodes the two ssDNA that will make the nanostructure, i.e. Oligos 1 and 5, from parts BBa_K2054001 and BBa_K2054005 respectively.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 1
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal NheI site found at 29
    Illegal NheI site found at 52
    Illegal NheI site found at 355
    Illegal NheI site found at 378
    Illegal NotI site found at 7
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 1
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 1
    Illegal XbaI site found at 16
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//chassis/prokaryote/ecoli
//dna
Parameters
chassisE. coli (DH10B)