DNA
O5

Part:BBa_K2054005

Designed by: LAI HEI WAI   Group: iGEM16_Hong_Kong_HKU   (2016-10-14)


Oligo 5 of DNA Tetrahedral Nanostructure

This will be used to build our desired nanostructure that target micro RNA biomarkers. This plasmid encodes the second ssDNA that will make the tetrahedral nanostructure, together with oligo 1, 2, 3 and 4.


Usage and Biology

Characterization conducted by:

  • Group: Team: HK_SKHLPSS, 2019
  • Author: Tse Cheong Tat
  • Summary: The peroxidase activity of the nano-structure formed from parts BBa_K2054001and BBa_K2054005 were measured. It was found that the nano-structure formed from these two parts had a high specificity in detecting the target sequence, showing that the purified ssDNAs produced from plasmid could work as per their design and function well. Details of our experimental characterization could be found on here.
  • Relevant documentation:
    • p1 p1

    Sequence and Features


    Assembly Compatibility:
    • 10
      COMPATIBLE WITH RFC[10]
    • 12
      INCOMPATIBLE WITH RFC[12]
      Illegal NheI site found at 7
      Illegal NheI site found at 30
    • 21
      COMPATIBLE WITH RFC[21]
    • 23
      COMPATIBLE WITH RFC[23]
    • 25
      COMPATIBLE WITH RFC[25]
    • 1000
      COMPATIBLE WITH RFC[1000]


[edit]
Categories
//chassis/prokaryote/ecoli
//dna
Parameters
chassisE. coli (DH10B)