DNA
Tetrahedro

Part:BBa_K2054000

Designed by: LAI HEI WAI   Group: iGEM16_Hong_Kong_HKU   (2016-10-14)


All Oligos for Tetrahedral Nanostructure

This will be used to build and express our desired functional nanostructure in E. coli that target micro RNA biomarkers. This plasmid encodes all the ssDNA that will make the tetrahedral nanostructure, i.e. Oligos 1, 2, 3, 4 and 5.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 1
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal NheI site found at 29
    Illegal NheI site found at 52
    Illegal NheI site found at 355
    Illegal NheI site found at 378
    Illegal NheI site found at 651
    Illegal NheI site found at 674
    Illegal NheI site found at 964
    Illegal NotI site found at 7
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 1
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 1
    Illegal XbaI site found at 16
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//chassis/prokaryote/ecoli
//dna
Parameters
chassisE. coli (DH10B)