Coding
DnaK

Part:BBa_K205002

Designed by: Michael James George Milevskiy   Group: iGEM09_UQ-Australia   (2009-09-28)


DnaK is a chaperone protein that assists with polypeptide folding

In conjuction with DnaJ and GrpE, DnaK assists in correct polypeptide folding and is the Prokaryotic homologue of the Eukaryotic chaperone system 'Hsp70'. This gene, along with DnaJ, GrpE, GroEL and GroES forms the heat shock system in bacteria.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 860
    Illegal PstI site found at 970
    Illegal PstI site found at 1321
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 860
    Illegal PstI site found at 970
    Illegal PstI site found at 1321
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 860
    Illegal PstI site found at 970
    Illegal PstI site found at 1321
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 860
    Illegal PstI site found at 970
    Illegal PstI site found at 1321
    Illegal NgoMIV site found at 156
    Illegal NgoMIV site found at 1671
    Illegal AgeI site found at 399
    Illegal AgeI site found at 871
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1588


[edit]
Categories
Parameters
None