Coding

Part:BBa_K2048004

Designed by: Seray Cicek   Group: iGEM16_Toronto   (2016-10-14)


Short GolS (P118)

This cpart contains Tet promoter, tetO and GolS(P118A) genes. GolS is a MerR family transcriptional regulator, gold-responsive activator of gol and ges genes. GolB is a promoter that gets activated by GolS factor. GolS(P118A) is the mutant of GolS that is more sensitive to gold.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 426
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None