Coding

Part:BBa_K2045002

Designed by: Thiban Navaratnarajah   Group: iGEM16_Concordia   (2016-10-14)


fhuA:Ferrichrome Iron outer membrane transporter

Ferrichrome Iron outer membrane transporter (FhuA) is a transmembrane protein endogenous to Escherichia coli cells. For the purposes of our project, we utilized FhuA's transmembrane properties fused to a Gold-binding peptide (GBP, see part BBa_K2045001), to attach our nanoparticles to the external surface of E.coli's cells. We consider this an improvement on iGEM BCCS-Bristol’s part, BBa_K259000. The fusion protein (FhuA-GBP) is submitted as BBa_K2045000.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 682
    Illegal AgeI site found at 820
    Illegal AgeI site found at 1225
    Illegal AgeI site found at 1999
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 724


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Categories
Parameters
None