This is the sequence of a D-lactate deshydrogenase that can efficiently use NADPH and NADH as cofactors. The enzyme is under control of a Pseudomonas putida constitutive promoter and RBS. This composite part was designed to produce lactate in order to increase lactate fraction used as substrate in the Ploylactic acid (PLA) production. The part ends with the BBa_B0015 terminator.
Sequence and Features
- 10COMPATIBLE WITH RFC
- 12COMPATIBLE WITH RFC
- 21Illegal BamHI site found at 45
- 23COMPATIBLE WITH RFC
- 25Illegal NgoMIV site found at 699
- 1000COMPATIBLE WITH RFC
Nucleotide sequence of a DNA segment promoting transcription in Pseudomonas putida.
Inouye S, Asai Y, Nakazawa A, Nakazawa T.
Engineering a d-lactate dehydrogenase that can super-efficiently utilize NADPH and NADH as cofactors.
Meng H1,2, Liu P3, Sun H2,3, Cai Z2, Zhou J2, Lin J3,4, Li Y2.