Pseudomonas resinovorans PHA synthase 1
This is the coding sequence for class II poly(R)-hydroxyalkanoic acid (PHA) synthase 1 from Pseudomonas resinovorans. The enzyme takes the monomer (D)-lactyl-CoA and produce the PLA homopolymer. This sequence is codon optimized for Pseudomonas putida and includes four amino acid mutations (E130D/S325T/S477G/Q481K) which were found to increase yields of PLA [1,2]. This type II PHA synthase 1 accept short-chain-length hydroxyacyl-CoAs including lactyl-CoA and 3-hydroxybutyryl-CoA as substrates and support the synthesis of P(3HB-co-LA). This biobrick correct Yale 2013 BBa_K1211002 biobrick. Indeed according to "http://www.ncbi.nlm.nih.gov/pubmed/21221571" the mutation on serine 477 is needed for PLA polymerization but in BBa_K1211002 this mutation is found on serine 475.
Sequence and Features
- 10COMPATIBLE WITH RFC
- 12COMPATIBLE WITH RFC
- 21Illegal BglII site found at 87
Illegal XhoI site found at 172
Illegal XhoI site found at 1030
- 23COMPATIBLE WITH RFC
- 25Illegal NgoMIV site found at 1060
- 1000Illegal BsaI site found at 796
 Biosynthesis of Polylactic Acid and Its Copolymers using Evolved Propionate CoA Transferase and PHA Synthase
Taek Ho Yang,1 Tae Wan Kim,1 Hye Ok Kang,1 Sang-Hyun Lee,1 Eun Jeong Lee,1 Sung-Chul Lim,1 Sun Ok Oh,1 Ae-Jin Song,2 Si Jae Park,1 Sang Yup Lee2,3
 Tailor-made type II Pseudomonas PHA synthases and their use for the biosynthesis of polylactic acid and its copolymer in recombinant Escherichia coli.
Yang TH1, Jung YK, Kang HO, Kim TW, Park SJ, Lee SY.
 Microbial production of lactate-containing polyesters.
Yang JE1, Choi SY, Shin JH, Park SJ, Lee SY.