Composite

Part:BBa_K2041016

Designed by: ZHOU CHUANGUI   Group: iGEM16_BIT   (2016-10-14)


FimE and Bxb1 device regulated by ptet and pbad

FimE recombinase can bind to its binding site and its level of expression is regulated by ptet.Bxb1 recombinase can bind to its binding site and its level of expression is regulated by pbad.These two recombinases will influent the level of expression of GFP.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1599
    Illegal NheI site found at 1822
    Illegal NheI site found at 3285
    Illegal NheI site found at 3308
    Illegal NheI site found at 3707
    Illegal NheI site found at 3730
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1539
    Illegal BamHI site found at 2096
    Illegal XhoI site found at 2183
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2735
    Illegal NgoMIV site found at 2822
    Illegal AgeI site found at 1872
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2930
    Illegal BsaI.rc site found at 4676


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