Composite

Part:BBa_K2041011

Designed by: ZHOU CHUANGUI   Group: iGEM16_BIT   (2016-10-14)


ptet-RBS-tetR-fimE-T-pbad-RBS-Bxb1-T

Use two promoters(ptet and pbad) to express fimE recombinase and Bxb1 recombinase respectively.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1599
    Illegal NheI site found at 1822
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1539
    Illegal BamHI site found at 2096
    Illegal XhoI site found at 2183
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2735
    Illegal NgoMIV site found at 2822
    Illegal AgeI site found at 1872
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2930


[edit]
Categories
Parameters
None