Composite

Part:BBa_K2041010

Designed by: ZHOU CHUANGUI   Group: iGEM16_BIT   (2016-10-14)


plac-RBS-Bxb1-T-plux-RBS-luxR-T-plux-recognition site of Bxb1-RBS-luxI-RBS-GFP-T

The Bxb1 recombinase can bind to the binding site of Bxb1 and make GFP express,but the level of expression is not high.Use an amplifying system (BBa_K546003) can increase the level of expression of GFP.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 418
    Illegal NheI site found at 3082
    Illegal NheI site found at 3105
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 4041
    Illegal BamHI site found at 692
    Illegal XhoI site found at 779
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1331
    Illegal NgoMIV site found at 1418
    Illegal AgeI site found at 468
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1526
    Illegal BsaI.rc site found at 1872
    Illegal BsaI.rc site found at 2975
    Illegal BsaI.rc site found at 4720


[edit]
Categories
Parameters
None