Composite

Part:BBa_K2041009

Designed by: ZHOU CHUANGUI   Group: iGEM16_BIT   (2016-10-14)


ptet-RBS-Bxb1-T

Use a ptet promoter(R0040),strong RBS(B0034),Bxb1 coding region(K907000),and a double terminator(B0015).Will generate Bxb1 recombinase when ptet begin to express.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 272
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 546
    Illegal XhoI site found at 633
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1185
    Illegal NgoMIV site found at 1272
    Illegal AgeI site found at 322
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1380


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Categories
Parameters
None