Composite

Part:BBa_K2041007

Designed by: YANG YUANZHAN   Group: iGEM16_BIT   (2016-10-14)


pbad-RBS-Bxb1-T

Use a pbad promoter(K206000),strong RBS(B0034),Bxb1 coding region(K907000),and a double terminator(B0015).Will generate Bxb1 recombinase when pbad begin to express.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 125
    Illegal NheI site found at 348
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 65
    Illegal BamHI site found at 622
    Illegal XhoI site found at 709
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1261
    Illegal NgoMIV site found at 1348
    Illegal AgeI site found at 398
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1456


[edit]
Categories
Parameters
None