Composite
Part:BBa_K2041007
Designed by: YANG YUANZHAN Group: iGEM16_BIT (2016-10-14)
pbad-RBS-Bxb1-T
Use a pbad promoter(K206000),strong RBS(B0034),Bxb1 coding region(K907000),and a double terminator(B0015).Will generate Bxb1 recombinase when pbad begin to express.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 125
Illegal NheI site found at 348 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 65
Illegal BamHI site found at 622
Illegal XhoI site found at 709 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1261
Illegal NgoMIV site found at 1348
Illegal AgeI site found at 398 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1456
[edit]
Categories
Parameters
None |