Composite

Part:BBa_K2037014

Designed by: Vaughn Murray Barendsen   Group: iGEM16_Pretoria_UP   (2016-10-14)


Laccase for in vitro expression

SP6 promoter joined to a tthl laccase from Thermus thermophilus with T7 promoter, RBS and His-tag

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1434
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 501
    Illegal NgoMIV site found at 988
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 816


[edit]
Categories
Parameters
None