Composite

Part:BBa_K2037009

Designed by: Ricu Claassens   Group: iGEM16_Pretoria_UP   (2016-10-12)


Laccase for in vitro expression

SP6 promoter attached to Laccase from Bacillus pumilus with His(6x) tag. T7 terminator at the end.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1621
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 182
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 549
    Illegal NgoMIV site found at 1017
    Illegal AgeI site found at 305
    Illegal AgeI site found at 1182
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None