Part:BBa_K2032001
Fructose Pathway
This composite biobrick part is a combination of the coding regions for three separate enzymes involved in the metabolization of methanol. Each coding region is preempted by a ribosome binding site in order to help counteract some of the translational issues associated with polycistronic mRNA. A lacI regulated promoter that allows for induction with IPTG was included in this construct to allow for selective gene expression along with the standard iGEM double terminator. The three enzymes encoded by this part are methanol dehydrogenase 2 (MDH), 3-hexulose-6-phosphate synthase (HPS), and 6-Phospho-3-hexuloisomerase (PHI). These enzymes serve to function as a three step pathway in which methanol is metabolized. MDH converts methanol to formaldehyde, producing a molecule of NADH in the process. Formaldehyde is then combined with a molecule of D-ribulose-5-phosphate taken from the pentose phosphate pathway to form one molecule of D-arabino-3-hexulose-6-phosphate via the usage of HPS. PHI then converts D-arabino-3-hexulose-6-phosphate to D-fructose-6-phosphate which can then undergo glycolysis.
(add detail)
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1405
Illegal BamHI site found at 1264 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1954
Illegal AgeI site found at 1290 - 1000COMPATIBLE WITH RFC[1000]
| None |