Composite

Part:BBa_K2032000

Designed by: Jacob Premo, Seth Cohen, Chun Mun Loke   Group: iGEM16_UMaryland   (2016-08-27)


sMMO

Contains the subunits necessary to assembly the soluble methane monooxygenase (sMMO) enzyme complex. Contains MMO subunits B, C, D, X, Y, and Z, each preceded by Anderson rbs B0032, and all under lactose of IPTG inducable control.

The sMMO complex oxidizes methane into methanol.

(add more detail later)

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 3792
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 3102
    Illegal BamHI site found at 3136
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 110
    Illegal NgoMIV site found at 1021
    Illegal NgoMIV site found at 1199
    Illegal NgoMIV site found at 3749
    Illegal NgoMIV site found at 4585
    Illegal AgeI site found at 3996
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1160
    Illegal BsaI site found at 1225
    Illegal BsaI.rc site found at 1268
    Illegal BsaI.rc site found at 1724
    Illegal BsaI.rc site found at 2111
    Illegal BsaI.rc site found at 2249
    Illegal BsaI.rc site found at 2666
    Illegal BsaI.rc site found at 3815


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Categories
Parameters
None