Composite

Part:BBa_K203104

Designed by: Michael Bartoschek   Group: iGEM09_Heidelberg   (2009-10-15)

mCherry-Myrpalm; PM targetted Red fluorescent protein

This part is a composition of both an mCherry fluorescent protein and a plasma membrane targetting sequence.

Myrcherry.jpg

Usage and Biology

One can use this part to stain the plasmamembrane of your cells. This enables you for example to investigate the transcription of two promoters if one is combined with this part and the other one with a differently localized fluorescent protein with an other color. Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 820
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 86
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 820
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 820
    Illegal AgeI site found at 49
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 46


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