The carboxylesterase (EstC2) is a catalytic enzyme preceded by secretion tag pP43_RBS_nprb. The used prefix pP43_RBS_nprb allows the secretion of EstC2 in B. subtilis.
Usage and Biology
This part can be used to produce and secrete EstC2 in B. subtilis. EstC2 can hydrolyse ester molecules into it's alcohol and acid.
Sequence and Features
- 10COMPATIBLE WITH RFC
- 12Illegal NheI site found at 381
- 21COMPATIBLE WITH RFC
- 23COMPATIBLE WITH RFC
- 25Illegal NgoMIV site found at 333
- 1000COMPATIBLE WITH RFC