Translational_Unit

Part:BBa_K2029012

Designed by: Bui Hoang Duy   Group: iGEM16_UBonn_HBRS   (2016-10-14)

nprb_CelA

The catalytic enzyme endo-1,4-beta-D-glucanase (CelA) cleaves 1,4-beta-D-glycosidic linkages in cellulose. The used prefix pP43_RBS_nprb allows the secretion of CelA in B. subtilis.

Usage and Biology

This part can be used to produce and secrete CelA in B. subtilis that can hydrolyse cellulose molecules into simple sugars.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 381
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 333
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None