Coding

Part:BBa_K2029007

Designed by: Bui Hoang Duy   Group: iGEM16_UBonn_HBRS   (2016-10-14)


EstC2

Catalytic enzyme (Esterase)

Part coding for a catalytic enzyme called carboxylesterase, which is used to hydrolyse esters and split it up into the alcohol and the corresponding acid. This specific gene sequence is naturally found in the microorganism Haliangium ochraceum.

Usage and Biology

It can be used to hydrolyse ester bonds of lipids found in commercial variaties of ink.

This gene is intended for use in B. subtilis, preceded by a secretory tag like BBa_K2029000 or BBa_K2029001 which signals the B. subtilis cell to secrete the coded protein into the medium.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 464
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 730
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 778


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Categories
Parameters
None