Coding

Part:BBa_K2029002

Designed by: Niklas Schmacke, Max Schelski   Group: iGEM16_UBonn_HBRS   (2016-10-14)


XynA

Part coding for the catalytic enzyme endo-1,4-beta-D-xylanase, which breaks down beta-1,4-xylan into xylose. This specific gene sequence is naturally found in some strains of Bacillus subtilis.

Usage and Biology

The enzymes expressed can be used to degrade hemicellulose, a major component of plant cell walls.

This gene is intended for use in B. subtilis, preceded by a secretory tag like BBa_K2029000 or BBa_K2029001 which signals the B. subtilis cell to secrete the coded protein into the medium.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None