Coding

Part:BBa_K2027056

Designed by: Gordon Sun   Group: iGEM16_Stanford-Brown   (2016-10-24)


E. coli DXS Synthase Enzyme cds (codon optimized)

This sequence codes for DXS synthase, an enzyme involved in the conversion of G3P and Pyruvate to DXP in the MEP/DOXP pathway. Because DXS synthase activity is the rate limiting step in the MEP/DOXP pathway, overexpression of this gene will override the bottleneck in the G3P and Pyr conversion process.

This sequence is endogenous to E. coli and is referenced from EcoGene:EG13612.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 257
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 134
    Illegal SapI.rc site found at 688


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Parameters
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