Composite

Part:BBa_K2027040

Designed by: Michael Becich   Group: iGEM16_Stanford-Brown   (2016-10-13)


DXS Synthase protein (G3P +Pyr->DXP)

The purpose of this construct is to optimize the conversion of pyruvate and G3P to DXP in the MEV/DOXP pathway. This construct can be either purposed for DXS synthase protein harvesting or optimization of the MEV/DOXP pathway with intent to harvest downstream products.

Through constitutive expression of DXS synthase (provided in this construct), the rate limiting step in pyruvate and G3P conversion to DXP can be overridden through increasing the amount of enzyme present. The DXS synthase protein also has a FLAG tag and Lumio His tag for visualization and nickel column purification.

Sequences were obtained from Zhao et al.(http://www.ncbi.nlm.nih.gov/pubmed/21468716), Accession number EG13612 in EcoGene. DNA sequences were codon optimized for E. coli expression.

This part contains part BBa_K2027056, which is the gene for DXS synthase (http://parts.igem.org/Part:BBa_K2027056).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 318
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 25
    Illegal SapI.rc site found at 749
    Illegal SapI site found at 195


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